Journal: BMC microbiology

Article Title: Lactobacillus Johnsonii YH1136 alleviates schizophrenia-like behavior in mice: a gut-microbiota-brain axis hypothesis study.

doi: 10.1186/s12866-025-03893-w

Figure Lengend Snippet: Fig. 2 The expression of related proteins in the hippocampus and prefrontal cortex (PFC) of mice. mRNA expression level of BDNF (a), TDO2 (b), IDO1 (c), IDO2 (d), KAT1 (e), KAT2 (f), KAT4 (g), KAT4 (h), TPH1 (i), NR1 (j), NR2A (k), and NR2B (l) in the Hippocampus and PFC. Data are expressed as mean ± SD (n = 5 per group). ns no significant, *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: Sections were then incubated overnight at 4 °C with polyclonal rabbit anti-TDO2 antibody (1:100, 15,880–1-AP, Proteintech), polyclonal rabbit anti-IDO1 antibody (1:100, 13,268–1-AP, Proteintech), polyclonal rabbit anti-IDO2 antibody (1:200, bs-16640R, Bioss), polyclonal rabbit anti-TPH1 antibody (1:50, BS3727, Bioworld), or polyclonal rabbit anti-KAT1 antibody (1:500, GB11906, Servicebio).

Techniques: Expressing

Journal: BMC microbiology

Article Title: Lactobacillus Johnsonii YH1136 alleviates schizophrenia-like behavior in mice: a gut-microbiota-brain axis hypothesis study.

doi: 10.1186/s12866-025-03893-w

Figure Lengend Snippet: Fig. 3 Immunofluorescence staining. The sections of the TDO2 (a-b), IDO1 (c-d), IDO2 (e–f), KAT1 (g-h), TPH1 (i-j) in hippocampus and PFC. The magnification is 20X (F)

Article Snippet: Sections were then incubated overnight at 4 °C with polyclonal rabbit anti-TDO2 antibody (1:100, 15,880–1-AP, Proteintech), polyclonal rabbit anti-IDO1 antibody (1:100, 13,268–1-AP, Proteintech), polyclonal rabbit anti-IDO2 antibody (1:200, bs-16640R, Bioss), polyclonal rabbit anti-TPH1 antibody (1:50, BS3727, Bioworld), or polyclonal rabbit anti-KAT1 antibody (1:500, GB11906, Servicebio).

Techniques: Immunofluorescence, Staining

Journal: iScience

Article Title: Amitriptyline revitalizes ICB response via dually inhibiting Kyn/Indole and 5-HT pathways of tryptophan metabolism in ovarian cancer

doi: 10.1016/j.isci.2024.111488

Figure Lengend Snippet: Expression of MYD88, IDO1, and IL4-I1 in EOC cells after LPS-induced combination with AMI (A−C) RT-qPCR and Western blot verification of MYD88 and IDO1 expression in A2780, OVCAR3, and ID8 EOC cells after LPS-induction (1 μg/ml). (D−F) mRNA expression of MYD88 and IDO1 by the combination of LPS with AMI in A2780, OVCAR3 (AMI: 50, 100μm) and ID8 (AMI: 30, 60μm) EOC cells. (G−H) MYD88 and IDO1 expression was not detected in A2780 EOC cells according to the pre-WB results. Therefore, only the expression of MYD88 and ID8 protein levels in OVCAR3 and ID8 were performed in the combined treatment. (Protein expression of MyD88 and IDO1 relative to Tubulin in EOC cells treated with different concentrations of drugs). (I) mRNA expression of IL4I1 on A2780, OVCAR3, and ID8 EOC cells after LPS combined with AMI treatment was analyzed by RT-qPCR, in which IL4I1, as a secreted protein, was detected in the levels in the EOC cells culture supernatant with Elisa. Each assay was performed in triplicate, and data are presented as mean ± SD ( n = 3).

Article Snippet: Antibody- IDO1 , Bioss , Cat# bs-15493R;.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Amitriptyline revitalizes ICB response via dually inhibiting Kyn/Indole and 5-HT pathways of tryptophan metabolism in ovarian cancer

doi: 10.1016/j.isci.2024.111488

Figure Lengend Snippet: 5-HT-mediated expression of IDO1 and PD-L1 after treatment with AMI and TGM2 inhibitors (A) Schematic diagram of the tryptophan-kynurenine metabolism pathway. (B and C) mRNA expression of IDO1 and PD-L1 in A2780, OVCAR3, and ID8 EOC cells exposed to different concentrations of 5-HT (50/100/200μM). (D−F) Protein expression of IDO1 and PD-L1 in A2780, OVCAR3, and ID8 EOC cells exposed to 5-HT (100μM). (G) AMI and TGM2 inhibitor (LDN-27219) were utilized respectively to inspect the mRNA expression of IDO1 and PD-L1 by A2780, OVCAR3, and ID8 EOC cells exposed to 5-HT (100μM). (H−J) Protein level expression of IDO1 and PD-L1 was detected in A2780, OVCAR3, and ID8 EOC cells exposed to 5-HT (100μM) using AMI and TGM2 inhibitor (LDN-27219), respectively. (protein expression of IDO1 and PD-L1 relative to GAPDH in EOC cells treated with different drugs) Each assay was performed in triplicate, and data are presented as mean ± SD ( n = 3).

Article Snippet: Antibody- IDO1 , Bioss , Cat# bs-15493R;.

Techniques: Expressing

Journal: iScience

Article Title: Amitriptyline revitalizes ICB response via dually inhibiting Kyn/Indole and 5-HT pathways of tryptophan metabolism in ovarian cancer

doi: 10.1016/j.isci.2024.111488

Figure Lengend Snippet: Expression of MyD88, AHR, and IDO1 in tumor tissues (A−C) Expression of MyD88, AHR, and IDO1 in tumor tissues of ID8 tumor-bearing mice after the above treatment by immunohistochemistry (The scale bar is 20μm, and the lower panel shows a 6× magnification view). (D−F) Expression of MyD88 , AHR, and IDO1 in tumor tissues of ID8 tumor-bearing mice after the above treatment by RT-qPCR. Data are presented as mean ± SD ( n = 3).

Article Snippet: Antibody- IDO1 , Bioss , Cat# bs-15493R;.

Techniques: Expressing, Immunohistochemistry, Quantitative RT-PCR

Journal: iScience

Article Title: Amitriptyline revitalizes ICB response via dually inhibiting Kyn/Indole and 5-HT pathways of tryptophan metabolism in ovarian cancer

doi: 10.1016/j.isci.2024.111488

Figure Lengend Snippet:

Article Snippet: Antibody- IDO1 , Bioss , Cat# bs-15493R;.

Techniques: Recombinant, Bicinchoninic Acid Protein Assay, Membrane, Isolation, Enzyme-linked Immunosorbent Assay, Lactate Dehydrogenase Assay, Software